Home Address:  2567 Barnwood Drive             
Birth Place: Portland, Oregon
Wexford, PA  15090

Home Phone:  (412) 849-4335                        
Citizenship: United States Citizen
Business Address:   204 Craft Avenue                    
E-mail Address: This email address is being protected from spambots. You need JavaScript enabled to view it.
Room B711
Pittsburgh, PA 15213
Business Phone:(412) 641-2460                        
Business Fax: (412) 641-3899

EDUCATION and TRAINING

UNDERGRADUATE

Dates Attended	Name and Location of Institution	Degree Received and Year	Major Subject
1986-1990	Whitworth College Spokane, Washington	B.S. B.A.	Biology Chemistry

GRADUATE

Dates Attended	Name and Location of Institution	Degree Received and Year	Major Advisor and Discipline
1990-1994	Oregon State University Corvallis, Oregon	Ph.D.	Fredrick Stormshak Biochemistry & Biophysics And Animal Sciences

POSTGRADUATE

Dates Attended	Name and Location of Institution	Name of Program Director and Discipline
1994-1998	University of Kansas Medical Center Kansas City, Kansas	Michael Soares Molecular and Integrative Physiology
1998-1999	School of Veterinary Medicine University of Pennsylvania Philadelphia, Pennsylvania	Ralph L. Brinster Animal Biology

APPOINTMENTS and POSITIONS

ACADEMIC:

Years Inclusive	Name and Location of Institution of Organization	Rank/Title
1999-2003	School of Veterinary Medicine University of Pennsylvania Philadelphia, PA	Research Assistant Professor of Physiology Department of Animal Biology
2003-2004	University of Pittsburgh School of Medicine Pittsburgh, PA	Visiting Assistant Professor Ob/Gyn & Reproductive Sciences And Molecular Genetics & Biochemistry
2004-2010	University of Pittsburgh School of Medicine Pittsburgh, PA	Assistant Professor OB/GYN & Reproductive Sciences and Molecular Genetics & Biochemistry
2003-Present	Magee-Womens Research Inst Pittsburgh, PA	Member
2004-Present	University of Pittsburgh School of Medicine Pittsburgh, PA	Graduate Faculty University of Pittsburgh School of Medicine
2008-Present	Magee-Womens Research Inst University of Pittsburgh SOM Pittsburgh, PA	Director Gene Editing, Transgenic and Virus Core (http://oorhs.pitt.edu/resources/genome-editing-transgenic-and-virus-core-getv)
2010-2015	University of Pittsburgh School of Medicine Pittsburgh, PA	Associate Professor with Tenure OB/GYN & Reproductive Sciences Secondary appointments in Microbiology and Molecular Genetics and Developmental Biology
2010-Present	University of Pittsburgh School of Medicine Pittsburgh, PA	Director of Research Reproductive Endocrinology and Infertility Division of OB/GYN & Reproductive Sciences
2010-Present	University of Pittsburgh Medical Center Pittsburgh, PA	Director Fertility Preservation Program of Pittsburgh (http://fertilitypreservationpittsburgh.org)
2011-2013	University of Pittsburgh School of Medicine Pittsburgh, PA	Vice Director Molecular Genetics and Development Biology Graduate Program
2013-2016	University of Pittsburgh School of Medicine Pittsburgh, PA	Director Molecular Genetics and Development Biology Graduate Program
2016-Present	University of Pittsburgh School of Medicine Pittsburgh, PA	Professor with Tenure OB/GYN & Reproductive Sciences Secondary appointments in Microbiology and Molecular Genetics; Developmental Biology; Clinical and Translational Science Institute

MEMBERSHIPS in PROFESSIONAL and SCIENTIFIC SOCIETIES

Organization	Year
Society for the Study of Reproduction	1991-present
International Society for Stem Cell Research	2004-present
American Society of Andrology	2005-present
American Society for Reproductive Medicine	2012-present
Society of Reproductive Biologists & Technologists	2012-present
Faculty of 1000	2013-present

HONORS

Organization	Year
Savory Outstanding Graduate Student, Oregon State University	1994
Sr. Vice Chancellor’s Seminar Series, invited speaker	2004
Recent Alumni Award and invited speaker, Whitworth College	2005
Fertility and Sterility STAR Reviewer	2014
Dozor Foundation Visiting Scholar, Ben Gurion University of the Negev, Israel	2016
Chair, Cellular, Molecular and Integrative Reproduction (CMIR) Study Section, NIH	2017-2019
Vice Chair, Gordon Conference on Mammalian Reproduction, Tuscany	2018
ASRM Star Award	2018

PUBLICATIONS

Refereed Articles

1. Orwig KE, Leers-Sucheta S, Moghaddam MF, Jiang ZD, Gerwick WR, and Stormshak F. Unique Metabolites of Eicosapentaenoic Acid Interfere With Corpus Luteum Function in the Ewe. Prostaglandins 1992; 44:519-530.
2. Orwig KE, Bertrand JE, Ou BR, Forsberg NE, and Stormshak F. Immunochemical Characterization and Cellular Distribution of Protein Kinase C Isozymes in the Bovine Corpus Luteum. Comp.Biochem.Physiol.Biochem.Mol.Biol. 1994; 108:53-57.
3. Orwig, KE, Bertrand, JE, Ou, BR, Forsberg, NE, and Stormshak, F. Involvement of Protein Kinase-C, Calpains, and Calpastatin in Prostaglandin F2 Alpha-Induced Oxytocin Secretion From the Bovine Corpus Luteum. Endocrinology 1994; 134:78-83.
4. Stormshak F, Orwig KE, and Bertrand JE. Dynamics of Molecular Mechanisms Underlying Ovarian Oxytocin Secretion. J.Reprod.Fertil.Suppl. 1995; 49:379-390.
5. Rasmussen CA, Hashizume K, Orwig KE, Xu L, and Soares MJ. Decidual Prolactin-Related Protein: Heterologous Expression and Characterization. Endocrinology 1996; 137:5558-5566.
6. Soares, MJ, Chapman, BM, Rasmussen, CA, Dai, G, Kamei, T, and Orwig, KE. Differentiation of Trophoblast Endocrine Cells. Placenta. 1996; 17:277-289.
7. Orwig KE, Ishimura R, Muller H, Liu B, and Soares MJ. Identification and Characterization of a Mouse Homolog for Decidual/Trophoblast Prolactin-Related Protein. Endocrinology 1997; 138:5511-5517.
8. Orwig KE, Dai G, Rasmussen CA, and Soares MJ. Decidual/Trophoblast Prolactin-Related Protein: Characterization of Gene Structure and Cell-Specific Expression. Endocrinology 1997; 138:2491-2500.
9. Rasmussen CA, Orwig KE, Vellucci S, and Soares MJ. Dual Expression of Prolactin-Related Protein in Decidua and Trophoblast Tissues During Pregnancy in Rats. Biol.Reprod. 1997; 56:647-654.
10. Dai G, Chapman BM, Liu B, Orwig KE, Wang D, White RA, Preuett B, and Soares MJ. A New Member of the Mouse Prolactin (PRL)-Like Protein-C Subfamily, PRL-Like Protein-C Alpha: Structure and Expression. Endocrinology 1998; 139:5157-5163.
11. Muller H, Orwig KE, and Soares MJ. Identification of Two New Members of the Mouse Prolactin Gene Family. Biochim.Biophys.Acta 1998; 1396:251-258.
12. Muller H, Ishimura R, Orwig KE, Liu B, and Soares MJ. Homologues for Prolactin-Like Proteins A and B Are Present in the Mouse. Biol.Reprod. 1998; 58:45-51.
13. Soares MJ, Muller H, Orwig KE, Peters TJ, and Dai G. The Uteroplacental Prolactin Family and Pregnancy. Biol.Reprod. 1998; 58:273-284.
14. Orwig KE and Soares MJ. Transcriptional Activation of the Decidual/Trophoblast Prolactin-Related Protein Gene. Endocrinology 1999; 140:4032-4039.
15. Shinohara T, Orwig KE, Avarbock MR, and Brinster RL. Spermatogonial Stem Cell Enrichment by Multiparameter Selection of Mouse Testis Cells. Proc. Natl. Acad. Sci. U.S.A. 2000; 97:8346-8351.
16. Nagano M, Brinster CJ, Orwig KE, Ryu BY, Avarbock MR, and Brinster RL. Transgenic Mice Produced by Retroviral Transduction of Male Germ-Line Stem Cells. Proc. Natl. Acad. Sci. U.S.A. 2001; 98:13090-13095.
17. Shinohara T, Orwig KE, Avarbock MR, and Brinster RL. Remodeling of the Postnatal Mouse Testis Is Accompanied by Dramatic Changes in Stem Cell Number and Niche Accessibility. Proc. Natl. Acad. Sci. U.S.A. 2001; 98:6186-6191.
18. Orwig KE, Avarbock MR, and Brinster RL. Retrovirus-Mediated Modification of Male Germline Stem Cells in Rats. Biol.Reprod. 2002; 67:874-879.
19. Orwig KE, Ryu BY, Avarbock MR, and Brinster RL. Male Germ-Line Stem Cell Potential Is Predicted by Morphology of Cells in Neonatal Rat Testes. Proc.Natl.Acad.Sci.U.S.A. 2002; 99:11706-11711.
20. Orwig KE, Shinohara T, Avarbock MR, and Brinster RL. Functional Analysis of Stem Cells in the Adult Rat Testis. Biol.Reprod. 2002; 66:944-949.
21. Shinohara T, Orwig KE, Avarbock MR, and Brinster RL. Germ Line Stem Cell Competition in Postnatal Mouse Testes. Biol.Reprod. 2002; 66:1491-1497.
22. Shinohara T, Orwig KE, Avarbock MR, and Brinster RL. Restoration of Spermatogenesis in Infertile Mice by Sertoli Cell Transplantation. Biology of Reproduction 2003; 68:1064-1071.
23. Ryu BY, Orwig KE, Avarbock MR, and Brinster RL. Stem Cell and Niche Development in the Postnatal Rat Testis. Developmental Biology 2003; 263:253-263.
24. Brinster CJ, Ryu BY, Avarbock MR, Karagenc L, Brinster RL, and Orwig KE. Restoration of Fertility by Germ Cell Transplantation Requires Effective Recipient Preparation. Biology of Reprod. 2003; 69:412-420.
25. Costoya JA, Hobbs RM, Barna M, Cattoretti G, Manova K, Sukhwani M, Orwig, KE, Wolgemuth DJ, Pandolfi PP. Essential role of Plzf in maintenance of spermatogonial stem cells.  Genet. 2004; 36:653-659.
26. Ryu BY, Orwig KE, Kubota H, Avarbock MR, Brinster RL. Phenotypic and functional characteristics of spermatogonial stem cells in rats.  Biol. 2004; 274:158-170.
27. Buageaw A, Sukhwani M, Ben-Yehudah A, Ehmcke J, Rawe VY, Pholpramool C, Orwig KE, Schlatt S. GDNF family receptor alpha1 phenotype of spermatogonial stem cells in immature mouse testes.  Reprod. 2005; 73:1011-1016.
28. Orwig, KE and Schlatt, S.   Cryopreservation and transplantation of spermatogonia and testicular tissue for preservation of male fertility.  Journal of the National Cancer Institute Monographs.  34:51-56.
29. Ryu BY, Orwig KE, Oatley JM, Avarbock MR, Brinster RL. Effects of Aging and Niche Microenvironment on Spermatogonial Stem Cell Self-Renewal.  Stem Cells 2006; 24:1505-1511.
30. Ryu BY, Orwig KE, Oatley JM, Lin CC, Chang LJ, Avarbock MR, Brinster RL. Efficient generation of transgenic rats through the male germline using lentiviral transduction and transplantation of spermatogonial stem cells. J. Androl  2007; 28: 353-360.
31. Hermann BP, Sukhwani M, Lin CC, Sheng Y, Tomko J, Rodriguez M, Shuttleworth JJ, McFarland D, Hobbs RM, Pandolfi PP, Schatten GP, Orwig KE. Characterization, cryopreservation and ablation of spermatogonial stem cells in adult rhesus macaques.  Stem Cells  2007; 25: 2330-2338.
32. Navara CS, Mich-Basso JD, Redinger CJ, Ben-Yehudah A, Jacoby E, Kovkarova-Naumovski E, Sukhwani M, Orwig KE, Kaminski N, Castro CA, Simerly CR, Schatten G. Pedigreed Primate Embryonic Stem Cells, Express Homogeneous Familial Gene Profiles.  Stem Cells 2007; 25: 2695-2704.
33. Yue J, Sheng, Y, Orwig KE. Identification of novel homologous microRNA genes in the rhesus macaque genome.  BMC Genomics 2008; 9: 8.  This article received the “Highly Accessed” designation from BMC Genomics.
34. Orwig KE, Ryu BY, Master S, Phillips BT, Mack M, Avarbock MR, Brinster RL. Genes Involved in Post-transcriptional Regulation are Over Represented in Stem/progenitor Spermatogonia of Cryptorchid Mouse Testes. Stem Cells 2008; 26: 927-938.
35. Escamilla-Hernandez, R, Little-Ihrig L, Orwig KE, Yue J, Chandran U, Zeleznik AJ. Constitutively Active Protein Kinase A Qualitatively Mimics the Effects of Follice-Stimulating Hormone on Granulosa Cell Differentiation. Mol Endocrinol 2008; 22: 1842-52.
36. Hermann BP, Sukhwani M, Simorangkir DR, Plant TM, Orwig KE. Molecular dissection of the male germ cell lineage identifies putative spermatogonial stem cells in rhesus macaques.  Human Reproduction 2009; 24: 1704-1716.
37. Sheng Y, Lin CC, Yue J, Sukhwani M, Shuttleworth JJ, Chu T, Orwig KE. Generation and characterization of a Tet-On (rtTA-M2) transgenic rat.  BMC Dev Bio 2010; 10:17.
38. Ben-Yehudah A, Easley CA 4^th, Hermann BP, Castro C, Simerly C, Orwig KE, Mitalipov S, Schatten G. Systems biology discoveries using non-human primate pluripotent stem and germ cells: novel gene and genomic imprinting interactions as well as unique expression patterns.  Stem Cell Res Ther 2010; 1:24.
39. Simerly C, McFarland D, Castro C, Lin CC, Redinger C, Jacoby E, Mich-Basso J, Orwig K, Mills P, Ahrens E, Navara C, Schatten G. Interspecies chimera between primate embryonic stem cells and mouse embryos: Monkey ESCs engraft into mouse embryos, but not post-implantation fetuses.  Stem Cell Res 2011; 7:28-40.
40. Hermann BP, Sukhwani M, Salati J, Sheng Y, Chu T, Orwig KE. Separating spermatogonia from cancer cells in contaminated prepubertal primate testis cell suspensions.  Human Reproduction. 2011; 26:3222-31.
41. Clark AT, Phillips BT and Orwig KE. Fruitful progress to fertility: Male fertility in the test tube. Nat Med. 2011; 17:1564-5.
42. Suzuki H, Ahn HW, Chu T, Bowden W, Gassei K, Orwig K, Rajkovic A. SOHLH1 and SOHLH2 coordinate spermatogonial differentiation. Dev Biol. 2012; 361:301-12.
43. Easley CA, Phillips BT, McGuire MM, Barringer JM, Valli H, Hermann BP, Simerly CR, Rajkovic A, Miki T, Orwig KE, Schatten GP. Direct differentiation of human pluripotent stem cells into haploid spermatogenic cells. Cell Reports 2012; 3:440-6.
44. Hermann BP, Sukhwani M, Winkler F, Pascarella JN, Peters KA, Sheng Y, Valli H, Rodriguez M, Esselarab M, Dargo G, Peterson K, Masterson K, Ramsey C, Ward T, Lienesch M, Volk A, Cooper DK, Thomson AW, Kiss JE, Penedo MT, Schatten GP, Mitalipov S and Orwig KE. Spermatogonial stem cell transplantation into Rhesus testes regenerates spermatogenesis producing functional sperm. Cell Stem Cell 2012 (Featured article); 11:715-726.**

**Community Choice Highlight in Nature 491: 641, The Most Viewed Papers in Science.

45. Gassei K and Orwig KE. SALL4 expression in gonocytes and spermatogonial clones of postnatal mouse testes.  PLoS ONE 2013; 8:e53976.
46. Dovey SL, Valli H, Hermann BP, Sukhwani M, Donohue J, Castro CA, Chu T, Sanfilippo JS, Orwig KE. Eliminating malignant contamination from therapeutic human spermatogonial stem cells. Journal of Clinical Investigation 2013; 123:1833-1843.
47. Skaznik-Wikiel ME, McGuire MM, Sukhwani M, Donohue J, Chu T, Krivak TC, Rajkovic A, Orwig KE. Granulocyte colony-stimulating factor with or without stem cell factor extends time to premature ovarian insufficiency in female mice treated with alkylating chemotherapy.  Fertil Steril 2013; 99:2045-2054.
48. Shetty G, Uthamanthil RK, Zhou W, Shao SH, Weng CC, Tailor RC, Hermann BP, Orwig KE and Meistrich ML. Hormone Suppression with GnRH Antagonist Promotes Spermatogenic Recovery from Transplanted Spermatogonial Stem Cells in Irradiated Cynomolgus Monkeys. Andrology 2013; 1:886-898.
49. Zhang Z, Zhang S, Huang X, Orwig KE, Sheng Y. Rapid assembly of customized TALENs into multiple delivery systems. PLoS ONE. 2013; 8:e80281.
50. Valli H, Phillips BT, Shetty G, Byrne JA, Clark AT, Meistrich ML, Orwig KE. Germline stem cells: toward the regeneration of spermatogenesis. Fertil Steril 2014; 101:3-13.
51. Puri P, Phillips BT, Suzuki H, Orwig KE, Rajkovic A, Lapinski PE, King PD, Feng G-S and Walker WH. The transition from stem cell to progenitor spermatogonia and male fertility requires the SHP2 protein tyrosine phosphatase. Stem Cells 2014; 32: 741-753.
52. Durruthy Durruthy J, Ramathal C, Sukhwani M, Fang F, Cui J, Orwig KE, Reijo Pera RA. Fate of induced pluripotent stem cells following transplantation to murine seminiferous tubules. Hum Mol Gen 2014; 23: 3071-3084.
53. Ramathal C, Durruthy-Durruthy J, Sukhwani M, Arakaki JE, Turek PJ, Orwig KE, Reijo Pera RA. Fate of iPSCs derived from azoospermic and fertile men following xenotransplantation to murine seminiferous tubules.  Cell Reports 2014; 7: 1284-1297.
54. Valli H, Sukhwani M, Dovey SL, Peters KA, Donohue JN, Chu T, Marshall GR, Orwig KE. Fluorescence- and Magnetic-activated cell sorting strategies to isolate and enrich human spermatogonial stem cells. Fertil Steril 2014; 102: 566–580.
55. Gassei K, Valli H, Orwig KE. Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans. Methods Mol Biol. 2014; 1210: 193–202.
56. Medrano JV, Martinez-Arroyo AM, Martinez-Jabaloyas JM, Pellicer A, Remohi J, Orwig KE, Simon C. Germ cell transplantation into mouse testis procedure (Video Submission).  Fertil and Steril 2014, 102: e11-e12.
57. Dominguez AA, Chiang HR, Sukhwani M, Orwig KE, Reijo Pera RA. Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies. Sci Rep. 2014; 4: 6432.
58. Ramathal C, Angulo B, Sukhwani M, Cui J, Durruthy-Durruthy J, Fang F, Schanes P, Turek PJ, Orwig KE, Reijo Pera R. DDX3Y gene rescue of a Y chromosome AZFa deletion restores germ cell formation and transcriptional programs. Sci Rep. 2015; 5:15041.
59. Pech M, Garbuzov A, Hasegawa K, Sukhawani M, Zhang RJ, Benayoun BA, Brockman SA, Lin S, Brunet A, Orwig KE and Artandi SE. High telomerase is a hallmark of undifferentiated spermatogonia and is required for maintenance of male germline stem cells. Genes and Development 2015; 29:2420-2434.
60. Toocheck C, Clister T, Shupe J, Crum C, Ravindranathan P, Lee TK, Ahn JM, Raj GV, Sukhwani M, Orwig KE, Walker WH. Mouse Spermatogenesis Requires Classical and Nonclassical Testosterone Signaling. Biol Reprod. 2016; 94:11, 1–14.
61. Gassei K and Orwig KE. Experimental methods to preserve male fertility and treat male factor infertility. Fertil and Steril 2016;105:256–66.
62. Simerly C, Castro C, Hartnett C, Lin CC, Sukhwani M, Orwig K, Schatten G. Post-Testicular Sperm Maturation: Centriole Pairs, Found in Upper Epididymis, are Destroyed Prior to Sperm's Release at Ejaculation. Sci Rep 2016; 6:31816.
63. Song HW, Bettegowda A, Lake BB, Zhao AH, Skarbrevik D, Babajanian E, Sukhwani M, Shum EY, Phan MH, Plank TD, Richardson ME, Ramaiah M, Sridhar V, de Rooij DG, Orwig KE, Zhang K, Wilkinson MF. The Homeobox Transcription Factor RHOX10 Drives Mouse Spermatogonial Stem Cell Establishment. Cell Reports 2016; 17(1):149-64.
64. Panula S, Reda A, Stukenborg JB, Ramathal C, Sukhwani M, Albalushi H, Edsgard D, Nakamura M, Soder O, Orwig KE, Yamanaka S, Reijo Pera RA, Hovatta O. Over Expression of NANOS3 and DAZL in Human Embryonic Stem Cells. PLos One 2016; 11(10):e0165268.
65. Sosa E, Kim R, Rojas EJ, Hosohama L, Hennebold JD, Orwig KE, Clark AT. An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts. Stem Cell Res 2016; (17) 2:444-447.
66. Johnson EK, Finlayson C, Rowell EE, Gosiengfiao Y, Pavone ME, Lockhart B, Orwig KE, Brannigan RE, Woodruff TK. Fertility Preservation for the Pediatric Patients: Current State and Future Possibilities. Urol. 2017; S0022-5347(17)30223-9.
67. Singh D, Paduch DA, Schlegel PN, Orwig KE, Mielnik A, Bolyakov A, Wright WW. The production of glial cell line-derived neurotrophic factor by human sertoli cells is substantially reduced in sertoli cell-only testes. Hum Reprod 2017; March 23: 1-10. PMID: 28369535
68. Gassei K, Sheng Y, Fayomi A, Mital P, Sukhwani M, Lin C-C, Peters KA, Althouse A, Valli H, Orwig KE. Ddx4-egfp transgenic rat model for the study of germline development and spermatogenesis. Biology of Reproduction 2017; 96:707-719.
69. Clark AT, Gkountela S, Chen D, Liu W, Sosa E, Sukhwani M, Hennebold JD, Orwig KE. Primate primordial germ cells acquire transplantation potential by Carnegie stage 23. Stem Cell Reports 2017; 9:1-13. PMID: 28579394
70. Sosa E, Kim R, Rojas EJ, Hosohama L, Hennebold JD, Orwig KE, Clark AT. An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts. Stem Cell Res 2017; 21:5-8. PMID: 28677539
71. Jakus AE, Laronda MM, Rashedi AS, Robinson CM, Lee C, Jordan SW, Orwig KE, Woodruff TK, Shah RN. Tissue Papers from Organ-Specific Decellularized Extracellular Matrices. Advanced Functional Materials 2017; 27:1-4. PMID: 29104526
72. Garbuzov A, Pech MF, Hasegawa K, Sukhwani M, Zhang RJ, Orwig KE, Artandi SE. Purification of GFRa1+ and GFRa- Spermatogonial Stem Cells Reveals a Niche-Dependent Mechanism for Fate Determination. Stem Cell Reports 2018; 10:553-567. PMID: 29337115
73. Fang F, Angulo B, Xia N, Sukhwani M, Wang Z, Carey CC, Mazurie A, Cui J, Wilkinson R, Wiedenheft B, Irie N, Surani MA, Orwig KE, Reijo Pera RA. A pax5–oct4–prdm1 developmental switch specifies human primordial germ cells. Nature Cell Biology 2018. PMID:29713018
74. Murdock MH, David S, Swinehart IT, Reing JE, Tran K, Gassei K, Orwig KE and Badylak SF. Human testis extracellular matrix enhances human spermatogonial stem cell survival in vitro. Tissue Engineering Part A. 2018, doi: 10.1089/ten.TEA.2018.0147.
75. Shetty G, Mitchell JM, Lam TNA, Wu Z, Zhang J, Hill L, Tailor RC, Peters KA, Penedo MC, Orwig KE, Meistrich ML. Donor spermatogenesis in de novo formed seminiferous tubules from transplanted testicular cells in rhesus monkey testis. Hum Reprod. 2018; 33(12):2249-2255.
76. Shetty G, Wu Z, Lam TNA, Phan TT, Orwig KE, Meistrich ML. Effect of hormone modulations on donor-derived spermatogenesis or colonization after syngeneic and xenotransplantation in mice. Andrology 2018; doi: 10.1111/andr.12571.
77. Smith BM, Duncan FE, Ataman L, Smith K, Quinn GP, Chang RJ, Finlayson C, Orwig K, Valli-Pulaski H, Moravek MB, Zelinski MB, Irene Su H, Vitek W, Smith JF, Jeruss JS, Gracia C, Coutifaris C, Shah D, Nahata L, Gomez-Lobo V, Appiah LC, Brannigan RE, Gillis V, Gradishar W, Javed A, Rhoton-Vlasak AS, Kondapalli LA, Neuber E, Ginsberg JP, Muller CH, Hirshfeld-Cytron J, Kutteh WH, Lindheim SR, Cherven B, Meacham LR, Rao P, Torno L, Sender LS, Vadaparampil ST, Skiles JL, Schafer-Kalkhoff T, Frias OJ, Byrne J, Westphal LM, Schust DJ, Klosky JL, McCracken KA, Ting A, Khan Z, Granberg C, Lockart B, Scoccia B, Laronda MM, Mersereau JE, Marsh C, Pavone ME, Woodruff TK. The National Physicians Cooperatve: transforming fertility management in the cancer setting and beyond. Future Oncol. 2018; doi: 10.2217/fon-2018-0278.
78. Sosa E, Chen D, Rojas EJ, Hennebold JD, Peters KA, Wu Z, Lam TN, Mitchell JM, Sukhwani M, Tailor RC, Meistrich ML, Orwig KE, Shetty G, Clark AT. Differentiation of primate primordial germ cell-like cells following transplantation into the adult gonadal niche. Nat Commun. 2018;9(1):5339. doi: 10.1038/s41467-018-07740-7.

Books, Book Chapters and Reviews:

 

1. Soares, MJ, Dai, G, Cohick, CB, Müller, H and Orwig, KE.   The rodent placental prolactin family and pregnancy.  In:  Bazer FW (ed) Endocrinology of Pregnancy.  Humana Press Inc., Totowa, NJ.   pp 145-176.
2. Orwig KE, Rasmussen CA, and Soares MJ. Decidual Signals in the Establishment of Pregnancy: the Prolactin Family. Trophoblast Research 1997; 10:329-343.
3. Orwig KE, Wolfe MW, Cohick CB, Dai G, Peters TJ, and Soares MJ. Trophoblast-Specific Regulation of Endocrine-Related Genes. Trophoblast Research 1998.
4. Filley S, Supancic S, Salli U, Orwig KE, and Stormshak F. Myristoylated Alanine-Rich C Kinase Substrate Protein and MRNA in Bovine Corpus Luteum During the Estrous Cycle. Endocrine. 2000; 12:289-294.
5. Hermann BP, Sukhwani M, Hansel MC and Orwig KE. Spermatogonial stem cells in higher primates: are there differences from those in rodents? Reproduction 2010; 139: 479–493.
6. Phillips BT, Gassei K, and Orwig KE. Spermatogonial stem cell regulation and spermatogenesis.  Phil Trans R Soc B 2010; 365, 1663-1678.
7. Hermann BP and Orwig KE.   Translating spermatogonial stem cell transplantation to the clinic.  In: KE Orwig and BP Hermann (eds.) Male Germline Stem Cells: Developmental and Regenerative Potential. Stem Cell Biology and Regenerative Medicine. New York: Humana Press.  227-253.
8. Hermann BP, Phillips BT and Orwig KE. The Elusive Spermatogonial Stem Cell Marker? Biol. Reprod. 2011; 85:221-3.
9. Sheng Y and Orwig KE. 2012. The roles of small RNAs in germ cell development and reproduction. miRNAs and Human Diseases. Kerala, India: Research Signpost. 257-291.
10. Valli H, Phillips BT, Gassei K, Nagano MC, Orwig KE.   Spermatogonial stem cells and spermatogenesis. In: T Zeleznik and TP Plant (eds.) The Physiology of Reproduction.  Philadelphia: Elsevier.  595-635.
11. Valli H, Gassei K, Orwig KE.   Stem cell therapies for male infertility: Where are we now and where are we going?  In: DT Carrell, PN Schlegel, C Racowsky, L Gianaroli (eds.) Biennial Review of Infertility.  New York: Springer. 17-39.
12. Gassei K, Orwig KE. Experimental Methods to Preserve Male Fertility and Treat Male Infertility. Fertil Steril 2016;105:256–266.
13. Gassei K, Shaw PH, Cannon GM, Meacham LR, Orwig KE. Male fertility preservation: Current options and advances in research. In: Woodruff TK, Gosiengfiao YC, eds. Pediatric and adolescent oncofertility: Best practices and emerging technologies. Chapter 8: Springer International Publishing; 2017:119-142.
14. Johnson EK, Finlayson C, Rowell EE, Gosiengfiao Y, Pavone ME, Lockart B, Orwig KE, Brannigan RE, Woodruff TK. Fertility preservation for pediatric patients: Current state and future possibilities. J Urol 2017;198:186-194.
15. David S and Orwig KE. Fertility Preservation in Cancer Patients. In: Oatley JM, Griswold MD, eds. The Biology of Mammalian Spermatogonia. Chapter 13: Springer Nature; 2017: 315-341.
16. Fayomi AP, Orwig KE. Spermatogonial stem cells and spermatogenesis in mice, monkeys and men. Stem Cell Res 2018; 29:207-214. PMID:29730571
17. Woodruff TK. Oncofertility research and practice: a multidisciplinary approach. Cham: Springer Nature; 2019. Chapter title: Male Fertility Preservation – Current Options and Advances in Research

Authors: Kathrin Gassei, Hanna Valli-Pulaski, Allison G. Close, Erika D. Friehling, Rajeev Chaudhry, Patrick J. Fox, Glenn M. Cannon, Thomas M. Jaffe, Lillian R. Meacham, Kyle E. Orwig.

 

PROFESSIONAL ACTIVITIES

Teaching:

Course Title	# Students	Type	Contact Hours	# Lectures	Years Taught
BI 395 Human Anatomy, Whitworth College	25	Undergraduate	6 hr/week	10	1989
ANS 327 Applied Physiology of Reproduction, Oregon State University	20	Undergraduate	3 hours	3	1992
ANS 317 Reproduction in Domestic Animals, Oregon State University	22	Undergraduate	2 hr/week	10	1993
PHSL 834 Reproductive Physiology, University of Kansas Medical Center	12	Graduate	1	1	1996
MSCMP 3740, Stem Cells, Interdisciplinary Graduate Program, University of Pittsburgh SOM	~20	Graduate	2	1	2004-2015, 2017
Reproductive and Developmental Biology, University of Pittsburgh SOM	~10	Medical students	14.5	9	2005-2006
Reproductive Endocrinology Research and Training Conference, University of Pittsburgh SOM	~10	Residents, Medical students Graduate	1	1	2005, 2006, 2008
MSIMM 2240, Immunobiotherapeutics, Interdisciplinary Graduate Program, University of Pittsburgh SOM	~5	Graduate	1	2	2005-2010, 2013, 2016, 2017, 2018
BIOE 1002, Intramural internship, Bioengineering, University of Pittsburgh	1	Undergraduate	108 (9 hrs/wk x 12 weeks)	Internship	2007
MED 5222, Reproductive and Developmental Biology, University of Pittsburgh SOM	~150	Medical Students	1	1	2008-2013, 2015-2018
Fundamentals in Bench Research Summer Course, University of Pittsburgh SOM	~20	Medical Fellows	1	1	2008-2014
MED 5115, Human Genetics, University of Pittsburgh SOM	8	Medical Students	16	8	2008, 2010
Scholarly Project – Jenn Salati	1	MS-4	>100	-	2008
MSCBMP 2880, Cell Biology, Interdisciplinary Graduate Program, University of Pittsburgh SOM	10	Graduate	3	2	2009
EPIDEM 2720, Reproductive Epidemiology, Graduate School of Public Heath, University of Pittsburgh	12	Graduate	1	1	2010, 2013, 2014, 2016, 2018
MSCMP 3770, Cell Therapy, Interdisciplinary Graduate Program, University of Pittsburgh SOM	~10	Graduate	1.5	1	2010-2013 2015-2016
MSCBMP 3840, Reproductive Development¸ Interdisciplinary Graduate Program, University of Pittsburgh SOM	10	Graduate	3	2	2011-2012, 2015, 2017
MSMGDB 2535, Model Organisms, Interdisciplinary Graduate Program, University of Pittsburgh, SOM	4	Graduate	2	1	2012-2018
MSMGDB 2550, Research Seminar (Course Director)	13	Graduate	1	13	2012-2013
MSCMP 2730, Molecular Mechanisms	15	Graduate	1	1	2016, 2017, 2018
CSCS, Changing Science Changing Society	35	Medical	3	2	2017-2018

 

 

Graduate Students Trained:

Bart Phillips, PhD rotation student Fall 2005, PhD dissertation student 2005-2012

Kathrin Gassei, PhD student, 2008-2009

Marc Hansel, PhD rotation student, Spring 2009

Mahesh Raundhal, PhD rotation student, Fall 2009

Andrew Hertsenberg, PhD rotation student, Summer 2010

Hanna Valli, PhD rotation student, Fall 2010, PhD dissertation student 2010-2014

Jihee Sohn, PhD rotation student, Spring 2011

Andrea Braganza, PhD rotation student, Fall 2011

Adetunji Fayomi, PhD rotation student Spring 2013, PhD dissertation student 2013-present

Hwa Fine Han, PhD rotation student, Fall 2014

Chatchanan Doungkamchan, rotation student, Spring 2015, PhD dissertation student 2015-present

Sherin David, PhD rotation student, Spring 2015, PhD dissertation student 2015-present

Jonathan Heier, PhD rotation student, Summer 2015

Katrina Harris, PhD rotation student, Fall 2015

Pinar Sahin, Turkish visiting scholar PhD dissertation student 2015-2017

Sarah Munyoki, PhD rotation student, Fall 2016, PhD dissertation student 2017-present

Michelle Scribner, PhD rotation student, Fall 2016

Lin Lin, PhD student, Xiangya University Chinese visiting scholar, 2016-2018

Kevin Peasley, PhD rotation student, Spring 2017

Julia Loose, PhD rotation student, Spring 2017

Kien Tran, PhD rotation student, Spring 2018

Xiang Yang, PhD student, Xiangya University Chinese visiting scholar, 2017-present

Nilgun Yersal, Turkish visiting scholar PhD dissertation student 2018-present

 

Graduate Students Mentored:

Meghan Sullivan, PhD student, first year mentee, 2013-2014

Adetunji Fayomi, PhD student, first year mentee, 2013-2014

Hwa Han, PhD rotation student Fall 2014, first year mentee, 2014-2015

Chatchanan Doungkamchan, PhD student, first year mentee, 2014-2015

Paige Davison, PhD student, first year mentee, 2014-2015

Shelby Hemker, PhD student, first year mentee, 2014-2015

Helen Rich, PhD student, first year mentee, 2014-2015

Jacqueline Welty, PhD student, first year mentee, 2014-2015

John Heir, PhD rotation student Summer 2015

Katrina Harris, PhD rotation student Fall 2015

Asan Alparslan, PhD student, first year mentee, 2015-2016

Kasey Cargill, PhD student, first year mentee, 2015-2016

Sarah Dremel, PhD student, first year mentee, 2015-2016

Nicolas Smith, PhD student, first year mentee, 2015-2016

Graduate Exam Committees:

Matt Boyer, PhD Student, Comprehensive Exam, August 2006

Matt Boyer, PhD Student, Thesis Committee, 2007-2011

Bart Phillips, PhD Student, Chair, Thesis Committee, 2007-2012

Rohan Manohar, PhD Student, Comprehensive Exam, July 2008

Rohan Manohar, PhD Student, Thesis Committee, 2008-2012

Marc Hansel, PhD Student, Thesis Committee, 2010-2012

Hanna Valli, PhD Student, Chair, Thesis Committee, 2012-2014

Adetunji Fayomi, PhD Student, Chair, Thesis Committee, 2016-2018

Michael Buckenmeyer, PhD Student, Thesis Committee, 2016-present

Hwa Han, PhD Student, Comprehensive Exam, August 2016

Hwa Han, PhD Student, Thesis Committee, 2016-present

Chatchanan Doungkamchan, PhD Student, Chair, Thesis Committee, 2016-present

Sherin David, PhD Student, Chair, Thesis Committee, 2016-present

Sarah Munyoki, PhD Student, Thesis Committee, 2018-present

Julia Loose, PhD Student, Thesis Committee, 2018-present

Kevin Peasley, PhD Student, Thesis Committee, 2018-present

 

Postdoctoral Fellows Trained:

Buom-Yong Ryu, Postdoctoral fellow, 2000-2003

Yi Sheng, Postdoctoral fellow, 2005-2008

Meena Sukhwani, Postdoctoral fellow, 2003-2007

Brian Hermann, Postdoctoral fellow, 2005-2011

Kathrin Gassei, Postdoctoral fellow, 2009-2013

Minjung Yoon, Postdoctoral fellow, 2011-2012

Payal Mital, Postdoctoral fellow, 2014-2016

Amanda Zielen, Postdoctoral fellow, 2018-present

 

Clinical Fellows Trained:

Serena Dovey, REI, 2008-2011

Malgorzata Skaznik-Wikiel, Gyn-Onc, 2010-2011

Melanie Ochalski, REI, 2009-2012

Shruti Malik, REI, 2012-2014

Jessica Berger, Gyn-Onc, 2013-2015

Matrika Johnson, REI, 2013-2015

Stephanie Rothenberg, REI, 2017-present

Emily Barnard, REI, 2018-present

Clinical Residents Trained:

Melanie Ochalski, 2006-2009

Tiffany Beck, 2012-2013

Medical Students Trained:

Jennifer Salati, 2008-2009

Leticia Dwomor, 2016-present

 

Continuing Medical Education Activities:

Transgenesis in the male germ cell lineage.  Frontiers in Human Embryonic Stem cells Advanced Training Course.  Pittsburgh Development Center, Pittsburgh, PA, May 10, 2003.

Stem cells in the male germline.  Keystone Symposium on Stem Cells.  Keystone Resort, Keystone, Colorado.  January 26, 2004.

Male fertility after cancer treatment.  Fertility after Cancer mini-symposium, Frontiers in Human Embryonic Stem Cells Advanced Training Course, Pittsburgh Development Center, May, 2004.

Animal models of stem cell biology. American Association for Laboratory Animal Sciences, Ellicott City, MD. September 2004.

Biology and practical application of male germline stem cells.  Ob/Gyn Grand Rounds, University of Pittsburgh School of Medicine, Pittsburgh, PA  January 2005.

Fertility after Cancer.  Reproductive Endocrinology Research and Training Conference.  University of Pittsburgh School of Medicine, Pittsburgh, PA  March 2005.

Fertility after Cancer, Update.  Reproductive Endocrinology Research and Training Conference, University of Pittsburgh School of Medicine, Pittsburgh, PA  April 2006.

Stem cells.  15th Annual LHAS Medical Ethics Update, University of Pittsburgh School of Medicine, Pittsburgh, PA  April 2006.

Fertility options for cancer survivors.  Ob/Gyn Grand Rounds, University of Pittsburgh School of Medicine, Pittsburgh, PA  December 2007.

Fertility options for cancer survivors.  Grand Rounds, Children’s Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA  March 2008.

The totipotent cycle – germline development.  Reproductive endocrinology research and training conference.  University of Pittsburgh School of Medicine, Pittsburgh, PA  April 2008.

Recent progress studying spermatogonial stem cells in primates.  Society for the Study of Reproduction, 41^st Annual Meeting, Kona, Hawaii.  Invited Symposium Speaker.  May 28, 2008.

Male germline stem cells.  Frontiers in Human Embryonic Stem Cells Advanced Training Course.  Woods Hole, MA.  June 3, 2008.

Human Stem Cell Oversight at the University of Pittsburgh.  Pitt Research Network Seminar Series, University of Pittsburgh, Research Conduct and Compliance Office, Pittsburgh, PA.  October, 2008.

Cancer and Fertility.  UPMC Cancer Centers Professional Education, Shadyside Hospital, Pittsburgh PA.  January 13, 2009.

Oncology.  UPMC Cancer Centers Professional Education, Shadyside Hospital, Pittsburgh PA.  April 15, 2009.

Cancer and Fertility.  Internal Medicine Grand Rounds, Shadyside Hospital, University of Pittsburgh School of Medicine, Pittsburgh, PA.  May 7, 2009.

Fertility options for cancer survivors.  Ob/Gyn Grand Rounds, University of Pittsburgh School of Medicine, Pittsburgh, PA  June 2, 2009.

Male germline stem cells.  Frontiers in Human Embryonic Stem Cells Advanced Training Course.  Woods Hole, MA.  October, 2009.

Translating spermatogonial stem cell transplantation to the clinic.  MWRI Work in Progress Seminar.  Pittsburgh, PA.  December 1, 2009.

But my patient wants to have children. Third Annual Symposium on Hereditary Cancers.  Pittsburgh, PA.  December 3, 2009.

Cancer and Fertility.  Urology Grand Rounds, University of Pittsburgh School of Medicine, Pittsburgh, PA.  April 28, 2010.

Fertility Preservation Options for Adolescent and Young Adult Patients.  AYA Cancer Symposium. Children’s Hospital of Pittsburgh, PA.  April 29, 2010.

Male germline stem cells.  Frontiers in Human Embryonic Stem Cells Advanced Training Course.  Woods Hole, MA.  October 6, 2010.

Male germline stem cells.  Frontiers in Human Embryonic Stem Cells Advanced Training Course.  Woods Hole, MA.  October 2, 2011.

Sperm from infertility-patient stem cells.  Round Table Discussion, American Society for Reproductive Medicine 2011 Annual Meeting.  Orlando, FL.  October 17, 2011.

Translating spermatogonial stem cell transplantation to the clinic. Prostate and Urologic Cancer Program Seminar Series, Shadyside Hospital, University of Pittsburgh School of Medicine, Pittsburgh, PA.  December 19, 2011.

Translating spermatogonial stem cell transplantation to the clinic. Endocrine Grand Rounds, University of Pittsburgh Medical Center, Pittsburgh, PA.  March 30, 2012. Stem cells. 

Ethical Issues Surrounding Reproductive Technology in the field of Fertility Preservation. 21^st Annual Medical Ethics Update, University of Pittsburgh School of Medicine, Pittsburgh, PA  April 13, 2012.

Translating spermatogonial stem cell transplantation to the clinic. OB/GYN Grand Rounds, West Virginia University, Morgantown, WV.  April 19, 2012.

Fertility Preservation Options for AYA Patients. Adolescent & Young Adult (AYA) Oncology Symposium. Children’s Hospital of Pittsburgh, PA.  April 26, 2012.

Male germline stem cells.  Frontiers in Stem Cells in Cancer Advanced Training Course.  Howard University, Washington, DC.  May 4, 2012.

Preserving male fertility.  2012 Oncofertility Conference: Dialogues in Oncofertility.  Northwestern University, Chicago, Illinois.  September 26-28, 2012.

Biology and therapeutic potential of spermatogonial stem cells.  MWRI Work in Progress Seminar.  Pittsburgh, PA.  October 2, 2012.

Male germline stem cells.  Frontiers in Human Embryonic Stem Cells Advanced Training Course.  Woods Hole, MA.  October 4, 2012.

Fertility Preservation. Pediatric Endocrine Conference. Children’s Hospital of Pittsburgh, PA.  January 25, 2013.

Male fertility preservation and stem cell transplantation.  Frontiers in Stem Cells and Cancer.  Ponce School of Medicine, Ponce, PR.  February 8, 2013.

Issues Surrounding Fertility. Cancer Survivorship Issues for Healthcare Professionals. UPMC Cancer Center, Pittsburgh, PA.  March 23, 2013.

Bill Anderson Memorial Lecture: Preserving Male Fertility. American Association of Tissue Banks Spring 2013 Meeting. Tucson, AZ. April 7, 2013.

Translating Spermatogonial Stem Cell Transplantation Toward the Clinic. Frontiers in Reproduction. Woods Hole, MA. May 15, 2013.

Treating male infertility with spermatogonial stem cells.  Frontiers in Human Embryonic Stem Cells Advanced Training Course.  Woods Hole, MA.  October 3, 2013.

Cryopreservation of Reproductive Cells and Tissues: Real World Approaches and Laboratory Pearls. American Society for Reproductive Medicine Postgraduate Course. Boston, MA. October 12, 2013.

Reverse Translation: Fertility Preservation from the Bedside to the Bench.  MWRI Work in Progress Seminar.  Pittsburgh, PA.  March 11, 2014.

Translating Spermatogonial Stem Cell Transplantation Toward the Clinic. Frontiers in Reproduction. Woods Hole, MA. May 22, 2014.

Current and Ongoing Translational Research in Fertility Preservation. An overview of Fertility Preservation: Counseling tools, Current options, and Future directions in the pediatric and adult patient. Our Clubhouse. Pittsburgh, PA, September 10, 2014.

Preserving fertility of male cancer survivors.  2014 Oncofertility Conference: Bench to bedside, oncofertility advances in males and females.  Chicago, IL, September 22-23, 2014.

Preserving fertility of prepubertal and adolescent cancer patients.  Pediatrics Grand Rounds, University of South Dakota.  Sioux Falls, SD, September 25, 2014.

Frontiers in fertility preservation.  Ob/Gyn Grand Rounds, University of South Dakota.  Sioux Falls, SD, September 26, 2014.

KY Cha Symposium in Stem Cell Technology and Reproductive Medicine, American Society of Reproductive Medicine Annual Meeting, Honolulu, HI, October 21, 2014.

Stem cells, spermatogenesis and male fertility in higher primates. Frontiers in Reproduction. Woods Hole, MA. May 20, 2015.

Translating Spermatogonial Stem Cell Transplantation Toward the Clinic. Frontiers in Stem Cells and Regeneration. Woods Hole, MA. Oct. 1, 2015.

Stem Cell Approaches to Preserve and Restore Male Fertility: Challenges and Opportunities.  NICHD/ASRM Fertility Preservation Workshop. Baltimore, MD October 22, 2015.

Fertility Preservation for the Cancer Patient: An Update. Our Clubhouse. Pittsburgh, PA, November 19, 2015.

Ethical Issues in Reproductive Health. 25^th Annual Medical Ethics Update 2016. University of Pittsburgh, Pittsburgh, PA.  April 1, 2016.

Stem cells, spermatogenesis and male fertility in higher primates. Frontiers in Reproduction. Woods Hole, MA. May 17, 2016.

Translating Spermatogonial Stem Cell Transplantation Toward the Clinic. Frontiers in Stem Cells In Cancer. Woods Hole, MA. Sept. 29-30, 2016.

Fertility Preservation for the Cancer Patient: An Update. Our Clubhouse. Pittsburgh, PA, November 16, 2016.

Frontiers in Stem Cells in Cancer. Morehouse School of Medicine. Atlanta, GA. February 10, 2017.

Frontiers in Reproduction. Marine Biological Laboratory. Woods Hole, MA. May 14, 2017.

Frontiers in Stem Cells and Regeneration Course. MBL. Woods Hole, MA. October 11, 2017.

Fertility Preservation for Boys with Cancer. OB/GYN Grand Rounds. Pittsburgh, PA. November 28, 2017.

Standard and Experimental Approaches to Fertility Preservation in Young Patients. Pediatric Hematology/Oncology Grand Rounds. Pittsburgh, PA. September 11, 2018.

RESEARCH:

 

Current Grant Support:

Grant Number	Grant Title	Role in Project % Effort	Years Inclusive	Source $ Amount (Direct)
R01 HD092084	Improving Fertility Preservation in Boys with Cancer	PI, 10%	2017-2020	$1,023,902
R01 HD076412	Cellular Mechanisms of Chemotherapy-induced Male Infertility: Stem Cell or Niche?	PI, 30%	2014-2019	$2,752,520
P01 HD075795	Preserving Male Fertility After Cancer Therapy	PI, 35%	2014-2019	$5,334,688
R01 HD079546	Differentiating Human Embryonic Stem Cells Into Developing Germ Line	Sub Award PI	2014-2019	$90,648
R43 HD090888	Isochoric Pressure Assisted Vitrification of Testicular Tissue and Whole Testes	Sub Award PI	2017-2018	$60,818
T32 HD087194	Reproductive Development from Gonads to Fetuses	PI	2017-2022	$1,047,013
R21 HD079546	Molecular and Functional Hierarchy of Male Germline Stem Cell Differentiation	Sub Award PI	2017-2019	$37,162

Prior Grant Support:

Grant Number	Grant Title	Role in Project % Effort	Years Inclusive	Source $ Amount
R01 HD082065	Epigenetic Effects of Cisplatin on Gametes and Pre-Implantation Embryos	Sub Award PI	2015-2017	$40,000
Montana State University Subcontracted Funds	Human to Monkey Transplant	Sub Award PI	2014-2017	$225,000
United States – Israel Binational Science Foundation	Male Fertility Preservation	PI	2012-2016	$80,000
U54 HD068158	Stanford University for Reproductive and Stem Cell Biology	Collaborator	2011-2013	$50,000
R01 HD055475	Characterization of male germline stem cells in a rhesus model of infertility	PI, 50%	2008-2013	$1,907,447
R21 HD061289	New fertility preserving option for male cancer survivors	PI, 10%	2010-2012	$275,000
Richard King Mellon Foundation	Preserving Fertility in Patients with Cancer and Immunological Conditions that Require Bone Marrow Transplants-Including Boys and Girls	PI	2011-2012	$50,000
Scaife Family Foundation Pilot Project Grant	Processing and Cryopreservation of Ovarian Tissue	PI, 0%	2009-2011	$25,000
Magee-Womens Research Institute and Foundation matching funds	Fertility Preservation Program	PI, 0%	2009-2011	$25,000
U54 HD008610	Physiology and pathophysiology of the primate gonad: Project 1, Xenografting and organ culture of testicular tissue: two novel tools to study premeiotic germ cell development in men and monkeys.	PI, 11%	2008-2011	$376,659
U54 HD008610	Physiology and pathophysiology of the primate gonad: Project II, Developmental determinants of the spermatogenic ceiling in the testes of adult rhesus macaques.	Co-PI, 10%	2006-2011	$148,272
U54 HD008610	Physiology and pathophysiology of the primate gonad: ARRA Administrative Supplement	PI, 0%	2009-2011	$49,921
R01 AG024992	Aging of Male Germline Stem Cells	PI, 20%	2004-2009	$1,000,000
R01 RR18500	Male germline modifications in rats	PI, 50%	2003-2008	$1,250,000
R37 HD012913	Indo-US program on contraception and reproductive health research	Co-I, 10%	2005-2007	$150,000
PA Department of Health: Health Research Formula Fund	Fertility after cancer treatment	PI, 20%	2004-2005	$100,000
USDA Formula Funds	The transplantation of chicken spermatogonial stem cells as a mechanism to introduce genetic changes to enhance health and thereby productivity of chickens	10%	2001-2002	$12,000
USDA Formula Funds	The transplantation of chicken spermatogonial stem cells into mouse testis	10%	2000-2001	$6,000
Lalor Foundation Research Grant	Transcriptional regulation of the decidual/trophoblast prolactin-related protein	100%	1997-1998	$22,500
Lalor Foundation Research Grant	DPRP expression: Decidual trophoblast coordination	100%	1996-1997	$20,000
Kansas Health Foundation	Control of choriocarcinoma cell growth and differentiation Research and Training Postdoctoral Fellowship	100%	1995-1996	$18,000

Seminars and Invited Lectureships:

Spermatogonial stem cell transplantation and transgenesis.  International Symposium, Molecular Mechanisms of Development: From Stem Cells to Complex Cellular Networks.  Max-Planck Institute for Experimental Medicine, Göttingen, Germany.  October 10-11, 2002.

Transgenesis in the male germ cell lineage.  Frontiers in Human Embryonic Stem cells Advanced Training Course.  Pittsburgh Development Center, Pittsburgh, PA, May 10, 2003.

Stem cells in the male germline. Keystone Symposium on Stem Cells.  Keystone Resort, Keystone, Colorado.  January 26, 2004.

Stem cell engineering in the male germline. University of Pittsburgh Senior Vice Chancellor’s Research Seminar.  February 20, 2004.

Stem cells in the male germline.  Ottawa Health Research Institute Seminar Series.  Ottawa, Ontario.  April, 2004.

Fertility after cancer treatment.  Frontiers in Human Embryonic Stem Cells Advanced training course.  Pittsburgh Development Center, Pittsburgh, PA.  May 9, 2004.

Animal models for stem cell research.  American Association of Laboratory Animal Science.  Ellicott City, MD.  September 2004.

Biology and practical application of male germline stem cells.  Ob/Gyn Grand Rounds, Pittsburgh, PA.  January 2005.

Stem cells in the male germline.  Department of Molecular and Integrative Physiology Seminar Series, University of Kansas Medical Center, Kansas City, KS  March 2005.

Stem cells in the Male germline.  Stowers Institute for Medical Research, Kansas City, KS.  March 2005.

Stem cells in the male germline.  Frontiers in Human Embryonic Stem Cells Advanced Training Course.  Pittsburgh, PA.  May 26, 2005.

Male germline stem cells.  Stanford University, Palo Alto, CA, June 18, 2005.

What’s really at stake in stem cell research?  Whitworth College Homecoming Lecture.  Spokane, WA, September 15, 2005.

Male germline stem cell biology and application.  Specialized Cooperative Centers Program in Reproduction Research: Combined Ovarian and Male Reproduction Meeting.  Houston, TX.  March 9-10, 2006.

Stem Cells.  15th Annual LHAS Medical Ethics Update. University of Pittsburgh School of Medicine, Pittsburgh, PA.  April 24, 2006.

Biology of male germline stem cells.  Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania School of Veterinary Medicine, Kennett Square, PA.  April 25, 2006.

The aging niche and spermatogonial stem cell self renewal.  National Institute on Aging, Stem cells and aging meeting, Bolger Conference center, Potomac MD.  April 30-May 2, 2006.

Male germline stem cells and infertility.  American Scientific Affiliation 61^st Annual Meeting.  Calvin College, Grand Rapids, MI.  July 29, 2006.

Germ cell differentiation.  XII Argentine Congress on Reproductive Medicine.  Buenos Aires, Argentina, March 15-16, 2007.

Spermatogonial stem cells and fertility preservation.  XII Argentine Congress on Reproductive Medicine.  Buenos Aires, Argentina.  March 15-16, 2007.

Initial characterization of spermatogonial stem cells in the rhesus macaque.  NICHD Specialized cooperative centers program in reproduction and infertility research (SCCPIR).  Portland, OR.  May 14-15, 2007.

Transplantation assay to study rhesus spermatogonial stem cells.  Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD.  July 6, 2007.

Male germline stem cells.  Frontiers in Human Embryonic Stem Cells Advanced Training Course.  Pittsburgh, PA.  August 8, 2007.

Update on the characterization of spermatogonial stem cells in the monkey.  Male Focus Group Meeting, University of North Carolina School of Medicine, Chapel Hill, NC.  September 6-7, 2007.

Spermatogonial Stem Cells.  Department of Biochemistry & Cellular Biology. Georgetown University, Washington DC. February 5, 2008.

Recent progress studying spermatogonial stem cells in primates.  Society for the Study of Reproduction, 41^st Annual Meeting, Kona, Hawaii.  Invited Symposium Speaker.  May 28, 2008.

Male germline stem cells.  Frontiers in Human Embryonic Stem Cells Advanced Training Course.  Woods Hole, MA.  June 3, 2008.

Update on the characterization of spermatogonial stem cells in the monkey.  Male Focus Group Meeting, University of Washington, Seattle, WA.  September 17, 2008.

Human Stem Cell Oversight at the University of Pittsburgh.  Pitt Research Network Seminar Series, University of Pittsburgh, Research Conduct and Compliance Office, Pittsburgh, PA.  October 15, 2008.

Cancer and Fertility.  UPMC Cancer Centers Professional Education, Shadyside Hospital, Pittsburgh PA.  January 13, 2009.

Stem cells in the male germline.  Orthopedic Surgery department seminar.  University of Pittsburgh School of medicine, Pittsburgh, PA.  January 27, 2009.

Stem cells in the male germline.  Human Genetics seminar series, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA.  March 20, 2009.

Stem cells in the male germline.  Center for Reproductive Biology and Health.  Department of Dairy and Animal Science, Pennsylvania State University.  University Park, PA.  March 27, 2009.

Fertility and Oncology.  UPMC Cancer Centers Professional Education Seminar Series, Shadyside Hospital, Pittsburgh PA.  April 15, 2009.

Cancer and Fertility.  Breast Cancer Conference, Magee-Womens Hospital, Pittsburgh, PA.  April 16, 2009.

Stem cells in the male germline.  2009 Gordon Research Conference.  Mammalian Gametogenesis and Embryogenesis.  Waterville Valley, NH.  August 2-7th 2009.

Male germline stem cells.  Frontiers in Human Embryonic Stem Cells Advanced Training Course.  Woods Hole, MA.  October, 2009.

Developmental and regenerative potential of male germline stem cells. Department of Cellular and Structural Biology, University of Texas Health Science Center. San Antonio, TX. November 10, 2009.

Clonogenic pattern of spermatogonial expansion in primates.  SCCPIR Male Focus Group Meeting, The Jackson Laboratory, Bar Harbor, ME.  October 29-30, 2009.

But my patient wants to have children. Third Annual Symposium on Hereditary Cancers.  Pittsburgh, PA. December 3, 2009.

Spermatogonial stem cells: Potential for treating male infertility.  Urology Grand Rounds, University of Pittsburgh.  Pittsburgh, PA.  April 28, 2010.

Fertility Preservation Options for Adolescent and Young Adult Patients.  AYA Cancer Conference. Children’s Hospital of Pittsburgh, PA.  April 29, 2010.

Translating spermatogonial stem cell transplantation to the clinic.  Germline Preservation of Pediatric Cancer Patients: SSC Biology Workshop.  MD Anderson Cancer Center, Houston, TX.  June 1^st, 2010.

Spermatogenic lineage development in primates.  Male germline stem cell mini symposium. Marine Biological Laboratories, Woods Hole, MA.  October 4, 2010.

Male germline stem cells.  Frontiers in Human Embryonic Stem Cells Advanced Training Course.  Woods Hole, MA.  October 6, 2010.

Translating spermatogonial stem cells to the clinic. Department of Molecular Cell and Developmental Biology.  UCLA, Los Angeles, CA.  November 4, 2010.

Translating spermatogonial stem cells to the clinic. Department of Pediatric Endocrinology.  Children’s Hospital of Pittsburgh, PA.  January 14, 2011.

Male germline stem cells.  Frontiers in Stem Cells in Cancer Advanced Training Course.  Howard University, Washington, DC.  March 17, 2011.

Spermatogonial stem cells in primates: fundamental biology and clinical implications.  Oregon National Primate Research Center, Beaverton, OR.  April 22, 2011.

Spermatogonial stem cells to preserve and restore fertility in male cancer survivors: safety and feasibility. NICHD Fertility Preservation Meeting. Bethesda, MD. June 17, 2011.

Spermatogonial Stem Cells in Mice, Monkeys and Men. NICHD Stem Cells, Human Reproduction and Regenerative Medicine Conference. Bethesda, MD. June 20-21, 2011.

Preserving the fertility of prepubertal male cancer survivors.  Department of Cellular and Structural Biology Grand Rounds, UT Health Science Center. San Antonio, TX. July 18, 2011.

Male germline stem cells.  Frontiers in Human Embryonic Stem Cells Advanced Training Course.  Woods Hole, MA.  October 2, 2011.

Male germline stem cells.  Herrenhausen Symposium on Reproductive Biology.  Seeon, Germany.  October 4-7, 2011.

Sperm from infertility-patient stem cells.  Round Table Discussion, American Society for Reproductive Medicine 2011 Annual Meeting.  Orlando, FL.  October 17, 2011.

Translating spermatogonial stem cell transplantation to the clinic. Prostate and Urologic Cancer Program Seminar Series, Shadyside Hospital, University of Pittsburgh School of Medicine, Pittsburgh, PA.  December 19, 2011.

Spermatogonial stem cell transplantation restores male fertility.  St. Jude Children’s Research Hospital.  Memphis, TN.  January 13, 2012.

Translating spermatogonial stem cell transplantation to the clinic. Endocrine Grand Rounds, University of Pittsburgh Medical Center, Pittsburgh, PA.  March 30, 2012.

Ethical Issues Surrounding Reproductive Technology in the field of Fertility Preservation. 21^st Annual Medical Ethics Update, University of Pittsburgh School of Medicine, Pittsburgh, PA.  April 13, 2012.

Translating spermatogonial stem cell transplantation to the clinic. OB/GYN Grand Rounds, West Virginia University, Morgantown, WV.  April 19, 2012.

Fertility Preservation Options for AYA Patients. Adolescent & Young Adult (AYA) Oncology Symposium. Children’s Hospital of Pittsburgh, PA.  April 26, 2012.

Male germline stem cells.  Frontiers in Stem Cells in Cancer Advanced Training Course.  Howard University, Washington, DC.  May 4, 2012.

Translating Spermatogonial Stem Cell Transplantation Toward the Clinic. 58^th/60^th NIBB Conference.  Okazaki, Japan. July 17- 21, 2012.

Preserving male fertility.  2012 Oncofertility Conference: Dialogues in Oncofertility.  Northwestern University, Chicago, Illinois.  September 26-28, 2012.

Male germline stem cells.  Frontiers in Human Embryonic Stem Cells Advanced Training Course.  Woods Hole, MA.  October 4, 2012.

Translating Spermatogonial Stem Cell Transplantation to the Clinic. The 9^th Annual Gilbert S. Greenwald Symposium on Reproduction. Kansas City, MO. October 11-12, 2012.

Pediatric Endocrine Conference.  University of Pittsburgh School of Medicine.  January 25, 2013. 

Male fertility preservation and stem cell transplantation.  Frontiers in Stem Cells and Cancer.  Ponce School of Medicine, Ponce, PR.  February 8, 2013.

Preserving fertility of male cancer survivors.  Kansas State University, Manhattan, KS.  February 18, 2013.

Issues Surrounding Fertility. Cancer Survivorship Issues for Healthcare Professionals. UPMC Cancer Center, Pittsburgh, PA.  March 23, 2013.

Bill Anderson Memorial Lecture: Preserving Male Fertility. American Association of Tissue Banks Spring 2013 Meeting. Tucson, AZ. April 7, 2013.

Translating Spermatogonial Stem Cell Transplantation Toward the Clinic. XXII North American Testis Workshop. San Antonio, TX. April 13, 2013.

Translating Spermatogonial Stem Cell Transplantation Toward the Clinic. Frontiers in Reproduction. Woods Hole, MA. May 15, 2013.

Preserving Fertility with Spermatogonial Stem Cells. Gordon Conference on Germinal Stem Cell Biology.  Hong Kong. July 15, 2013.

Treating male infertility with spermatogonial stem cells.  Frontiers in Human Embryonic Stem Cells Advanced Training Course.  Woods Hole, MA.  October 3, 2013.

Male Germline Stem Cells. Rockefeller University, Population Council. New York, NY. December 6, 2013.

Male Fertility Preservation. Frontiers in Stem Cells and Cancer Advanced Training Course. Howard University. Washington, DC. December 13, 2013.

Human and Non-Human Primate Stem Cells. ASA Annual Meeting. Atlanta, GA. April 6, 2014.

Stem Cell Therapies for Male Infertility. Washington University, Department of Genetics. St. Louis, MO. May 1, 2014.

Stem Cells, Spermatogenesis and Male Fertility.  Frontiers in Reproduction. Woods Hole, MA. May 22, 2014.

Transplant technologies for assessing the regenerative potential of human stem cells.  Montana State University, Bozeman, MT, June 6, 2014.

Current and Ongoing Translational Research in Fertility Preservation. An overview of Fertility Preservation: Counseling tools, Current options, and Future directions in the pediatric and adult patient. Our Clubhouse. Pittsburgh, PA, September 10, 2014.

Preserving fertility of male cancer survivors.  2014 Oncofertility Conference: Bench to bedside, oncofertility advances in males and females.  Chicago, IL, September 22-23, 2014.

Preserving fertility of prepubertal and adolescent cancer patients.  Pediatrics Grand Rounds, University of South Dakota.  Sioux Falls, SD, September 25, 2014.

Stem cell therapies for male infertility.  Ob/Gyn Grand Rounds, University of South Dakota.  Sioux Falls, SD, September 26, 2014.

Translating Spermatogonial Stem Cell Transplantation to the Clinic. Frontiers in Stem Cells and Regeneration Advanced Training Course. Woods Hole, MA. October 1, 2014.

Stem cell therapies for male infertility. Science 2014, Session10: Stem Cells Remixed. University of Pittsburgh. Pittsburgh, PA. October 3, 2014.

Translating Spermatogonial Stem Cell Transplantation to the Clinic. Department of Urology Grand Rounds, University of Pittsburgh. Pittsburgh, PA. October 8, 2014.

Preserving fertility of boys with Cancer. SAYAO Oncofertility Think Tank.  University of California Irvine/Children’s Hospital of Orange County, Newport Beach, CA, October 9, 2014.

KY Cha Symposium in Stem Cell Technology and Reproductive Medicine, American Society of Reproductive Medicine Annual Meeting, Honolulu, HI, October 21, 2014.

Spermatogonial stem cells in higher primates.  School of Molecular Biosciences, Washington State University, Pullman, WA, November 20, 2014.

Advancing the frontiers of reproductive medicine at Magee.  Magee-Womens Hospital Ethics Committee Meeting, Pittsburgh, PA, December 18, 2014.

Male Fertility Preservation and Stem Cell Transplant. Frontiers in Stem Cells and Cancer.  Ponce School of Medicine, Ponce, PR.  February 13, 2015.

Chair: Healthy stem cells, Healthy Aging.  XXIII North American Testis Workshop.  Salt Lake City, Utah.  April 16, 2015.

Spermatogonial Stem Cell Transplantation for Male Fertility Preservation. 6^th International IVI Congress. Alicante, Spain. April 22-25, 2015.

Stem Cells, Spermatogenesis and Male Fertility.  Frontiers in Reproduction. Woods Hole, MA. May 20, 2015.

Assaying Spermatogenic Potential. Gordon Conference on Germinal Stem Cell Biology.  Hong Kong. June 1-5, 2015.

Stem Cell Therapies for Male Infertility. Vincent Center for Reproductive Biology, Harvard University, Boston, MA, June 8^th, 2015.

Chair: Stem Cells. American Society of Reproductive Medicine Annual Meeting. Baltimore, MD. October 17-21, 2015.

Stem Cell Approaches to Preserve and Restore Male Fertility: Challenges and Opportunities. NICHD Workshop: New Frontiers in Fertility Preservation Research: Considering the Broader Impact. Baltimore, MD. October 22, 2015.

Preserving Fertility of Childhood Cancer Patients. Division of Hematology/Oncology and the Lurie Cancer Center of Northwestern University’s Grand Rounds. Chicago, IL. November 6, 2015.

Preserving the Fertility of Women, Men, Girls and Boys Facing Gonadotoxic Therapies for Cancer and Other Conditions. 5^th Annual Womens Cancer Research Center Retreat. Pittsburgh, PA. November 13, 2015.

Fertility Preservation for the Cancer Patient: An Update. Our Clubhouse. Pittsburgh, PA, November 19, 2015.

Gene therapy in and around the germline: International Summit on Human Gene Editing: A Global Discussion. Washington, DC. Dec 1-3, 2015.

Sertoli Cell Gene Therapy. Gene Families and Isozymes Annual Meeting.  Placencia, Belize. December 6-11, 2015

Stem cell therapies for male infertility. Johns Hopkins Bloomberg School of Public Health. Baltimore, MD. Jan 4, 2016.

Stem cell therapies for male infertility. Cornell University Department of Biomedical Sciences. Ithaca, NY. February 23, 2016.

Spermatogonial stem cells: Ready for prime time? Society of Reproductive Investigation Annual Meeting. Montreal, QC. March 17, 2016.

Ethical Issues in Reproductive Health. 25^th Annual Medical Ethics Update 2016. University of Pittsburgh, Pittsburgh, PA.  April 1, 2016.

Stem Cells, Spermatogenesis and Male Fertility.  Frontiers in Reproduction. Woods Hole, MA. May 17, 2016.

Stem Cell Therapies for Male Infertility. Dozor Foundation Visiting Scholar. Fertility Preservation Workshop: Bench to Bedside.  Ben Gurion University, Beer Sheva, Israel. May 25, 2016.

Gene Therapies for Male Infertility.  Ob/Gyn Grand Rounds, Ben Gurion University, Beer Sheva, Israel.  May 26, 2016.

Stem cell therapies and gene therapies for male infertility. Cook-Regentec, Bloomington Indiana. September 15, 2016.

Translating Spermatogonial Stem Cell Transplantation Toward the Clinic. Frontiers in Stem Cells In Cancer. Woods Hole, MA. Sept. 29-30, 2016.

Regenerative Therapies for Treating Male Infertility. American Society for Reproductive Medicine Annual Meeting. Salt Lake City, UT. October 15-19, 2016.

Stem Cell Therapies for Male Infertility. SKLRB Symposium on Reproductive Biology. Beijing, China. October 24-27, 2016.

Reproductive Technologies Available to Preserve Fertility. Womens Health Conversations Annual Meeting. Pittsburgh, PA. November 3-5, 2016

Fertility Preservation for the Cancer Patient: An Update. Our Clubhouse. Pittsburgh, PA, November 16, 2016.

Frontiers in Stem Cells in Cancer. Morehouse School of Medicine. Atlanta, GA. February 10, 2017.

Germline Gene Therapy with Spermatogonial Stem Cells. Society of Reproductive Investigation. Orlando, FL. March 15-18, 2017.

Current and New Approaches in Fertility Preservation in Challenging Patients. College of Reproductive Biology and AAB Annual Conference. Houston, TX. May 18-19, 2017.

Sperm after autologous grafting of pre-pubertal testis tissue. NCTRI Male Research Focus Group Meeting. Bethesda, MD. May 23, 2017.

Stem Cell Therapies for Male Infertility. ASAS Annual Meeting. Baltimore, MD. July 11, 2017.

Stem Cell Therapies for Male Infertility. Kyoto University. Kyoto, Japan. July 24, 2017.

Gene Therapies for Male Infertility. International Research Symposium on Regulation of Germ Cell Development in vivo and in vitro. Kyushu University School of Medicine. Fukuoka, Japan. July 26-28, 2017.

Gene Therapy for Male Infertility. Canadian Fertility and Andrology Society. Vancouver, Canada. September 14-16, 2017.

Gene Therapies for Male Infertility. Reproductive Biology Seminar Series at UT South Western Medical Center. Dallas, TX. October 10, 2017.

Translating Spermatogonial Stem Cell Transplantation to the Clinic. Frontiers in Stem Cells and Regeneration Course. MBL. Woods Hole, MA. October 11, 2017.

Stem Cell Therapies for Male Infertility. ASRM Pre-Congress Course. San Antonio, TX. October 28, 2017.

Spermatogonial Stem Cell Transplantation for Fertility Preservation. ASRM Pre-Congress Course. San Antonio, TX. October 28, 2017.

Fertility Preservation for Boys with Cancer. Stanley Manne Children’s Research Institute Seminar Series. Chicago, IL. November 1, 2017.

Germline Gene Therapy Without Germline Transmission. MWRI Annual Research Retreat. Pittsburgh, PA. November 10, 2017.

Fertility Preservation for Boys with cancer: Testicular Tissue Grafting. Oncofertility Conference, Male Oncofertility Group Meeting, Chicago, IL. November 14, 2017.

Fertility Preservation for Boys with Cancer. OB/GYN Grand Rounds. Pittsburgh, PA. November 28, 2017.

Gene Therapies for Male Infertility. Reproductive Medicine Networks. Basking Ridge, NJ. December 19, 2017.

Stem Cell Therapies for Male Infertility. The Center for Organogenesis Seminar Series at University of Michigan School of Medicine. Ann Arbor, MI. January 23, 2018.

Gene Therapy for Spermatogenesis. Roundtable Discussion on Male Infertility: Research for New Male Infertility Treatment Approaches. Weill Cornell Medical Center, New York, NY. January 29, 2018.

Regenerative Therapies for Medically Induced Infertility. McGowan Institute Retreat. University of Pittsburgh. Pittsburgh, PA. March 5, 2018.

Stem Cell Therapies for Male Infertility. Department of Cellular Biology University of Georgia seminar series. Athens, GA. March 20, 2018.

From Stem Cells to Germ Cells: The Potential for Fertility Restoration. American Society of Andrology 43^rd Annual Conference. Portland, OR. April 21-24, 2018.

Frontiers in Stem Cells in Cancer. Xavier University. New Orleans, LA. May 9, 2018.

Frontiers in Reproduction. Spermatogenesis. Woods Hole, MA. May 14-15, 2018.

Stem Cell, Regenerative and Transplantation Medicine. NIH NHP Meeting on Biomedical Research. Bethesda, MD. August 23-24, 2018.

Standard and Experimental Approaches to Fertility Preservation in Young Patients. Pediatric Hematology/Oncology Grand Rounds. Pittsburgh, PA. September 11, 2018.

Translating Spermatogonial Stem Cell Transplantation to the Clinic. Frontiers in Stem Cells and Regeneration Course. MBL. Woods Hole, MA. October 4, 2018.

Gene Therapies for Male Infertility. American Society for Reproductive Medicine Annual Meeting. KY Cha Symposium. Denver, CO. October 6-9, 2018.

Gene Therapies for Infertility. Science 2018 Spotlight Session: Applications of Novel Molecular Tools. Pittsburgh, PA. October 19, 2018.

Gene Therapies for Male Infertility. Annual Research Day Michigan State University. East Lansing, MI. November 27, 2018.

Has Conventional IVF Reached Its Limits? Ovarian Club XII. Hong Kong. December 15-16, 2018.

Other Research Related Activities:

 

Editorial Positions:

            Journal of Endocrinology, Editorial Board, 2002-2009

            Veterinary Medicine International, Special Issue Editor, 2010-2012

Spermatogenesis, Editorial Board, 2010-2017

Stem Cell Research, Associate Editor, 2013-2018

Biology of Reproduction, Associate Editor, 2017-present

Journal Refereeing:

Biology of Reproduction

Molecular Endocrinology

Stem Cells and Cloning

Stem Cells

Stem Cell Research

Stem Cell Reports

Developmental Biology

Development

Journal of Endocrinology

Human Reproduction

Reproduction

FEBS Letters

Human Molecular Genetics

Journal of Reproduction and Fertility

Journal of Nutrition

Nature

Nature Medicine

Nature Genetics

Science

Cancer Research

Proceedings of the National Academy of Sciences

Endocrinology

Molecular Reproduction and Development

Transgenic Research

International Journal of Andrology

New England Journal of Medicine

Journal of Assisted Reproduction and Genetics

Cell

Cell Stem Cell

PLoS One

PLoS Genetics

            NIH Study Section Memberships:

2004               Development-1 study section, NIH

2004-2005      NICHD/ZHD1 Study Section, NIH

2004               ZRG1 EMNR-D Study Section, NIH

2005-2009      Standing member, ZRG1 GTIE Study Section, NIH

2006               Chair, Special Emphasis Panel for the NICHD GRASS committee

2008               UKGD Study Section, NIH

2009               RAG Study Section, NIH

2009-2013      Charter member, TAG Study Section, NIH

2013               NICHD ZHD1 DSR-L, U54 reproductive centers study section

2014               Chair, Special Emphasis Panel for CMIR EMNR-T(02) Study Section, NIH

2014                Member, PAR ZRG1 CB-J55R: "Improvement of Animal Models for Stem Cell-based Regenerative Medicine"

2015-present   Standing member, CMIR Study Section, NIH

2016                Chair, GGG-L(3), Member Conflict: Genetic Diseases Study Section, NIH

2017-present   Chair, CMIR Study Section, NIH

2017-present   Member, ZCA1 SRO-Q (01) NIH Anonymization Study

2018                Chair, ZRG1 VH-N (71) R - Large Animal Testing Centers for Evaluation of Somatic Cell Genome Editing Tools

            Extramural Grant Reviewing:

2004   USDA Competitive Research Grants Program

2005   Netherlands Organisation for Scientific Research, STW VIDI research project

2006   Israeli Ministry of Science and Technology Program in “Stem Cells"

2008   Northwest Genome Engineering Consortium, Pilot Project program

2008    New York Health Department and Empire State Stem Cell Board, NYSTEM program

2008    The Netherlands Organisation for Health Research and Development (ZonMw)

2008    The American Urogynecologic Society Basic Science Research Fellowship Award

2009    Medical Research Council Research Grant Program-UK

2015    The German Research Foundation

2015    ASCEND Pilot Projects – Morgan State University

2015    The Netherlands Organization for Health Research and Development (ZonMw)

2016    Chair: Ky Cha Award in Stem Cell Technology, ASRM

2016    Zika Virus Grant Review, ASRM

Intramural Grant Reviewing:

            2008    Pittsburgh Clinical and Translational Science Institute – Pilot Program

LIST of CURRENT RESEARCH INTERESTS:

Germline development

Stem cell biology

Functional genomics

Fertility/infertility

Gene therapy

SERVICE:

University and Medical School

2005                           Strategic Planning Committee, Magee-Womens Research Institute

2005                           Chair, Research Committee, Ob/Gyn & Reproductive Sciences

2006-2007                  Faculty Retreat Organizing Committee, Ob/Gyn & Reproductive Sciences

2006-2007                   MWRI Director Search Committee, Magee-Womens Research Institute, Ob/Gyn & Reproductive Sciences

2008-2010                   MWRI Web Committee, Magee-Womens Research Institute

2005-present              Steering Committee, Magee-Womens Research Institute

2005-present               Human Stem Cell Research Oversight Committee (hSCRO), University of Pittsburgh School of Medicine

2006-present              Postdoctoral Committee, Magee-Womens Research Institute

2009-present               Ethics Committee, Magee-Womens Hospital

2009-2012                   Recruitment Committee, Interdisciplinary Biomedical Graduate Program, University of Pittsburgh

2011-2016                   Steering Committee, Interdisciplinary Biomedical Graduate Program, University of Pittsburgh

2011-2013                   Vice Director, Molecular Genetics and Developmental Biology Graduate Program

2012-2013                   Course Director, MSMGDB 2550, Research Seminar

2013-2016                   Graduate Council, University of Pittsburgh School of Medicine

2013-2016                   Director, Molecular Genetics and Developmental Biology Graduate Program

2017-present               Non-Tenure Faculty Promotions and Appointments Committee

National/International

2004-2005                  Publication Committee, Society for the Study of Reproduction

2008-2009                  Local Arrangements Committee, Society for the Study of Reproduction

2011-2012                  Nominating Committee, Society for the Study of Reproduction

2011-2013                  Program Committee, 2013 Testis Workshop, San Antonio, TX

2012-2013                  Chair, Nominating Committee, Society for the Study of Reproduction

2012-present              Medical Advisory Committee, Reprotech

2014-2018                  Awards Committee, Society for the Study of Reproduction

2014-2015                   Scientific Program Committee for the 2015 Annual meeting of the American Society for Reproductive Medicine

2014-2015                   Program Committee for the 2016 Annual meeting of the American Society of Andrology

2015-2016                   Vice Chair, Regenerative Medicine and Stem Cell Biology Special Interest Group, American Society for Reproductive Medicine

2015-2016                   Program Committee for the 2017 Annual meeting of the American Society of Andrology

2015-2017                   Program Committee, Society for the Study of Reproduction 2017 Annual Meeting

2016-present               Research Committee, American Society for Reproductive Medicine

2016-present               Advisory Council, Alliance for Fertility Preservation (http://www.allianceforfertilitypreservation.org/)

2016-2017                   Chair, Regenerative Medicine and Stem Cell Biology Special Interest Group, American Society for Reproductive Medicine

2016-2018                   Co-Vice Chair, 2018 Gordon Conference on Mammalian Reproduction

2018-present               Co-Chair, 2020 Gordon Conference on Mammalian Reproduction